Fig. 3. Effect of acute administration of Pi on the duodenal NaPi-IIb protein abundance. The protein expression of NaPi-IIb was analysed in (BBMVs) from duodenal mucosa of rats intragastrically (A) or intravenously (B) infused with saline (NaCl) or with Pi (n≥ 6). β-tubulin was used as control for protein-loading. The Fig. shows representative blots and normalized NaPi-IIb/ β-tubulin expression. Columns in the graphs represent mean ± the standard error of the mean (SEM). Statistical comparisons were performed using student's t-test. *p<0.05 compared with the corresponding control value.